SpikeDB: DMS (Deep Mutational Scan) of Spike

SpikeDB provides data on changes in infectivity caused by mutations in the spike protein of the new coronavirus, as well as changes in antigenic escape, ACE2 affinity, and protein expression. These data are based on Deep Mutational Scan (DMS), a method of screening all possible point mutations in a given protein.

The new coronavirus (SARS-CoV2) pandemic continues due to the emergence of mutant strains. It is expected that new mutant strains will continue to appear in the future. The information in this database makes it possible to predict the risk of virus mutations, which is expected to inform future vaccine strategies and evaluation of therapeutic agents.

SpikeDB is operated by the Institute for Microbial Diseases, Osaka University.

ACE2 binding affinity

Immune escape

Omicron strain

The changes in infectivity due to amino acid mutations of the Omicron strain, first detected in South Africa, are shown on the left. The horizontal axis is the mutation site, and the vertical axis is the change in infectivity due to each amino acid substitution. Red indicates enhancement of infectivity and blue indicates attenuation. Eight of the 15 mutations in the RBD may increase infectivity, and mutations in the NTD region may also increase infectivity.